FastQCFastQC Report
Thu 2 Jun 2016
DRR015317_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015317_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14203878
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204840.1442141364492148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178470.12564878408558564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213050.051.29021
GTATCAA388550.039.7126921
ATCAACG469900.032.333363
TCAACGC471200.032.2344934
TATCAAC497300.030.665092
CAACGCA498150.030.5641335
AACGCAG522850.029.216016
GTGGTAT95600.025.7658251
ACGCAGA582550.025.7639477
CGCAGAG586200.025.3869368
GTACATG433550.024.2592531
TGGTATC100800.023.3548762
TACATGG432350.023.296852
GCAGAGT634050.022.8049379
ACATGGG452000.021.6606713
CAGAGTA620150.019.20022210-11
AGAGTAC590150.019.02365710-11
GAGTACT388900.018.97616212-13
CATGGGG280100.018.0538924
GTACTTT404550.017.83156414-15
Produced by FastQC (version 0.11.5)