FastQCFastQC Report
Thu 2 Jun 2016
DRR015317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14203878
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228230.16068147022946833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205260.14450983034351603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226050.053.724671
GTATCAA415500.039.3807031
ATCAACG505750.031.8249653
TCAACGC510200.031.5919694
CAACGCA529100.030.472075
TATCAAC533400.030.3069132
AACGCAG553800.029.1787036
GTGGTAT99850.027.6922841
TGGTATC100500.025.941082
ACGCAGA622200.025.6712387
CGCAGAG629400.025.1318198
GTACATG440450.022.5257591
GCAGAGT683900.022.5173979
TACATGG437900.021.715552
ACATGGG455250.019.966023
GAGTACT426000.019.46547712-13
CAGAGTA669750.019.34299710-11
AGAGTAC637150.019.1616410-11
GTACTTT441600.018.40873314-15
AGTACTT439600.017.7661512-13
Produced by FastQC (version 0.11.5)