FastQCFastQC Report
Thu 2 Jun 2016
DRR015316_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015316_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11717281
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316800.2703698921277044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281470.24021784576131613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160730.13717346200027122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA230200.054.9060861
GTATCAA423100.039.343181
ATCAACG525100.031.4054183
TCAACGC531400.031.0416584
TATCAAC545200.030.443232
CAACGCA554800.029.896875
GTGGTAT101700.029.1003841
AACGCAG581550.028.6938746
TGGTATC99550.028.5425222
ACGCAGA648850.025.4600817
CGCAGAG648150.025.3616648
GTACATG411450.024.433651
TACCTGG94750.024.317582
TACATGG408100.023.6324312
GCAGAGT692750.023.2635429
GTACCTG104550.023.1247391
TATAACG11700.022.5730972
TCTGTCG27350.022.1248138
ACATGGG414500.022.0284863
ATAACGC12000.021.2414933
Produced by FastQC (version 0.11.5)