FastQCFastQC Report
Thu 2 Jun 2016
DRR015316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11717281
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358270.305762062034699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330580.2821302996830067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186360.15904713730088063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244300.055.97451
GTATCAA453350.039.7591
GTGGTAT99850.031.8427331
ATCAACG563400.031.4185053
TGGTATC97400.031.296642
TCAACGC569700.031.0870344
TATCAAC582550.030.6384662
CAACGCA592100.029.9570675
AACGCAG621850.028.692156
TATAACG11600.027.8472462
ACGCAGA699150.025.2726027
CGCAGAG697000.025.220028
TACCTGG90450.023.792192
GTACATG413150.023.726711
ATAACGC13450.023.6764643
GCAGAGT746450.023.166119
TACATGG407950.023.1304282
ACATGGG416600.021.5778793
TAACGCA16800.021.3923724
GTACCTG105500.020.8975751
Produced by FastQC (version 0.11.5)