FastQCFastQC Report
Thu 2 Jun 2016
DRR015315_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015315_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13670414
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311300.22771804862676434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291010.21287577684187178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155380.11366151749317907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA379900.036.050021
GTATCAA588200.031.09381
ATCAACG699450.025.8025463
TCAACGC708700.025.5108224
TATCAAC724550.024.9926822
CAACGCA733400.024.6892495
AACGCAG760550.023.873736
GTACATG390200.022.7066231
TACATGG381750.022.018022
ACGCAGA826250.021.76047
CGCAGAG824100.021.6966728
ACATGGG393650.020.6166063
GCAGAGT867250.020.282229
GAGTACT523450.020.22163612-13
AGAGTAC700900.019.94498810-11
AGTACTT544700.018.51811212-13
GTACTTT570450.018.28836614-15
CAGAGTA865500.017.37410210-11
TCTGTCG35000.016.1188538
CATGGGG218500.015.9988014
Produced by FastQC (version 0.11.5)