FastQCFastQC Report
Thu 2 Jun 2016
DRR015315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13670414
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349710.25581522256750966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337910.2471834430178925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184000.1345972404347081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA403100.036.9377371
GTATCAA625100.031.2954941
ATCAACG748750.025.7799133
TCAACGC756200.025.5199154
TATCAAC773700.025.0923522
CAACGCA775650.024.838935
AACGCAG804300.024.044646
GTACATG383800.022.5484541
TACATGG376850.021.8283652
ACGCAGA882300.021.7024087
CGCAGAG880250.021.6495848
ACATGGG385850.020.503423
GAGTACT572250.020.33901212-13
AGAGTAC736950.020.22153910-11
GCAGAGT927600.020.1671989
TATAACG10750.019.4688682
GTACTTT615250.018.59703614-15
AGTACTT593800.018.5396112-13
CAGAGTA918800.017.51413510-11
TCTGTCG34850.016.4479968
Produced by FastQC (version 0.11.5)