FastQCFastQC Report
Thu 2 Jun 2016
DRR015314_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015314_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10311502
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290560.28178242122243685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264970.25696547408903186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148150.1436745102701818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205900.055.944071
GTATCAA384350.040.3348161
ATCAACG473800.032.2283173
TCAACGC477900.031.9327744
TATCAAC492050.031.1474272
CAACGCA495900.030.8018235
GTGGTAT85650.030.4059471
AACGCAG517850.029.6017026
TGGTATC87300.028.9464992
ACGCAGA576800.026.2577747
CGCAGAG580400.025.9548198
GCAGAGT611300.024.0852369
GTACATG338250.022.4785631
TACATGG334100.021.4781592
CAGAGTA602900.020.6852910-11
GAGTACT422650.020.54997412-13
ACATGGG339600.020.0361213
AGAGTAC580350.019.96418410-11
GTACTTT439250.019.57655514-15
AGTACTT428600.019.01208112-13
Produced by FastQC (version 0.11.5)