FastQCFastQC Report
Thu 2 Jun 2016
DRR015314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10311502
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326940.3170634113245578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309880.30051877990228776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175280.16998493526937203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218950.057.8297351
GTATCAA405150.039.964571
GTGGTAT83700.033.0848731
ATCAACG509050.031.3149283
TGGTATC87100.031.0799452
TCAACGC516600.030.8660744
TATCAAC526250.030.4926222
CAACGCA531450.029.9693585
AACGCAG554500.028.7891986
ACGCAGA619650.025.4319237
CGCAGAG623100.025.1012828
GCAGAGT652750.023.4525559
GTACATG337950.022.1160471
TACATGG335250.021.2793672
CAGAGTA643550.020.6534410-11
GAGTACT465750.020.31994212-13
AGAGTAC620350.019.94440710-11
ACATGGG344050.019.8872183
GTACTTT481950.019.3789414-15
AGTACTT471900.018.96559112-13
Produced by FastQC (version 0.11.5)