FastQCFastQC Report
Thu 2 Jun 2016
DRR015312_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015312_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15043487
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227120.15097563483785376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189610.1260412562592702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224200.050.364431
GTATCAA416000.040.288431
ATCAACG498450.033.1654363
TCAACGC505050.032.7599334
TATCAAC530250.031.3364812
CAACGCA543050.030.4680585
AACGCAG569300.029.1103826
ACGCAGA618700.026.3697267
CGCAGAG618350.026.068888
GTGGTAT106900.024.7034821
GTACATG460950.024.1212441
GCAGAGT674650.023.3407339
TACATGG454250.023.1866362
TGGTATC110000.022.8923032
ACATGGG475550.021.4718073
GAGTACT409250.019.51107612-13
CAGAGTA652550.019.46327410-11
AGAGTAC615750.019.3444910-11
CATGGGG293850.018.303974
GTACTTT434700.018.12254314-15
Produced by FastQC (version 0.11.5)