FastQCFastQC Report
Thu 2 Jun 2016
DRR015312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15043487
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263280.17501261509382765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223650.14866898877899784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243300.053.3430521
GTATCAA452100.040.2842941
ATCAACG560050.032.133683
TCAACGC563400.031.942614
TATCAAC588200.030.7681222
CAACGCA590950.030.453455
AACGCAG617850.029.2232846
GTGGTAT109850.026.0427671
ACGCAGA692500.025.7511867
CGCAGAG692900.025.5327958
GTACATG478400.024.4434341
TACATGG468250.023.8136962
TGGTATC113350.023.7608222
GCAGAGT742300.023.14729
ACATGGG486750.022.103253
GAGTACT472850.020.0110112-13
AGAGTAC687250.019.78622810-11
CAGAGTA722050.019.69579710-11
CATGGGG300700.019.3494264
GTACTTT501900.018.53237514-15
Produced by FastQC (version 0.11.5)