FastQCFastQC Report
Thu 2 Jun 2016
DRR015311_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015311_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11812020
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239820.20303047234935262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216080.1829323011644071No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119880.10148983831723955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197150.054.1806871
GTATCAA358700.040.0353471
ATCAACG446050.032.022013
TCAACGC453500.031.5261974
TATCAAC467550.030.5921692
CAACGCA474050.030.1984485
AACGCAG499850.028.738666
GTGGTAT89000.026.449281
TGGTATC86500.025.8485282
ACGCAGA555400.025.5735267
CGCAGAG553500.025.5560828
GTACATG334750.023.2996861
GCAGAGT592500.023.1909739
TACATGG334100.022.3394682
ACATGGG341750.021.0372713
GAGTACT406800.020.44531412-13
CAGAGTA586950.019.94135310-11
AGAGTAC559100.019.6244810-11
GTACTTT439900.018.64281814-15
AGTACTT423000.018.38769312-13
Produced by FastQC (version 0.11.5)