FastQCFastQC Report
Thu 2 Jun 2016
DRR015311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11812020
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272120.23037549885624983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253200.2143579167661416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143090.12113931402080254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214800.054.2781521
GTATCAA389700.039.785111
ATCAACG486500.031.5492423
TCAACGC492250.031.1807144
TATCAAC508750.030.3070372
CAACGCA506400.030.2825035
AACGCAG530950.029.0022546
GTGGTAT95450.026.7778641
TGGTATC96150.025.741032
ACGCAGA593650.025.6632357
CGCAGAG591800.025.6206848
GCAGAGT635650.023.2879739
GTACATG345700.022.9054811
TACATGG344900.021.50162
GAGTACT442900.020.46468412-13
ACATGGG348850.020.160323
AGAGTAC597450.020.13728310-11
CAGAGTA634150.020.02647410-11
AGTACTT459850.018.97814812-13
GTACTTT477250.018.87368414-15
Produced by FastQC (version 0.11.5)