FastQCFastQC Report
Thu 2 Jun 2016
DRR015310_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015310_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9477252
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233940.24684370532724043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213580.22536068472168938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118710.12525782790201212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168450.055.812111
GTATCAA308400.040.021611
ATCAACG388750.031.4293463
TCAACGC392750.031.0979964
TATCAAC401850.030.5258772
GTGGTAT71350.030.0542831
CAACGCA408150.029.9367285
TGGTATC69700.029.6590772
AACGCAG427700.028.6101386
ACGCAGA472350.025.602047
CGCAGAG470550.025.5357388
GCAGAGT511500.023.029099
GTACATG251300.022.2658271
TACATGG246400.021.6951142
GAGTACT371650.020.79890412-13
ACATGGG250000.020.6047763
CAGAGTA507450.020.24515310-11
AGAGTAC481800.019.95368410-11
TATAACG8250.019.3173982
GTACTTT411650.018.645114-15
Produced by FastQC (version 0.11.5)