FastQCFastQC Report
Thu 2 Jun 2016
DRR015310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9477252
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252050.26595262002107783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244590.2580811399760184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134850.14228808097537132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184100.057.1360171
GTATCAA335500.039.2686161
GTGGTAT78650.031.3935471
ATCAACG422850.030.8552883
TCAACGC426900.030.5732214
TGGTATC77100.030.2715152
TATCAAC438300.029.8426592
CAACGCA440800.029.5988165
AACGCAG460100.028.4758726
ACGCAGA516850.025.0411667
CGCAGAG514450.025.0076588
GCAGAGT552350.022.9624129
GTACATG260500.022.6990031
TACATGG258550.021.4853422
GAGTACT408250.020.86384412-13
ACATGGG261250.020.3909453
CAGAGTA546000.020.36381110-11
AGAGTAC520350.020.29661810-11
TATAACG8100.019.0969962
GTACTTT450150.018.77685714-15
Produced by FastQC (version 0.11.5)