FastQCFastQC Report
Thu 2 Jun 2016
DRR015309_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015309_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14044459
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241750.17213194185692735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196760.14009795606936515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221050.052.7510151
GTATCAA418350.041.5426451
ATCAACG518800.033.233043
TCAACGC523150.032.966584
TATCAAC539250.032.021572
CAACGCA562550.030.641925
AACGCAG594500.028.994426
ACGCAGA643850.026.342647
CGCAGAG641900.026.1121128
GTACATG510100.025.1088121
TACATGG506050.024.4219782
GCAGAGT691600.023.5390559
ACATGGG516300.022.7826333
GTGGTAT98750.022.5928341
TGGTATC98550.021.393842
AGAGTAC638850.019.7173510-11
GAGTACT404400.019.44727912-13
CAGAGTA677400.019.44156610-11
CATGGGG280600.018.8767074
AGTACTT416450.018.3164512-13
Produced by FastQC (version 0.11.5)