FastQCFastQC Report
Thu 2 Jun 2016
DRR015309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14044459
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285820.20351086503225224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240820.17146975899890482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140730.10020321893495505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237850.054.0432931
GTATCAA461000.041.943511
ATCAACG578450.033.1095663
TCAACGC583250.032.860484
TATCAAC598400.032.1981932
CAACGCA608800.031.4739275
AACGCAG644850.029.7990476
ACGCAGA714900.026.5737237
CGCAGAG709500.026.5002618
GTGGTAT102950.026.1928841
GTACATG530400.024.6643051
TGGTATC101400.024.4976442
TACATGG516550.024.3706132
GCAGAGT762650.024.1046479
ACATGGG527600.022.8237173
AGAGTAC710150.020.1347110-11
CAGAGTA742050.019.87302410-11
CATGGGG287050.019.5725144
GAGTACT474800.019.45838512-13
AGTACTT482950.018.96985212-13
Produced by FastQC (version 0.11.5)