FastQCFastQC Report
Thu 2 Jun 2016
DRR015308_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015308_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12821890
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218760.17061447259335402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182740.14252189029854412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238000.052.409981
GTATCAA446650.040.127171
ATCAACG543850.032.689613
TCAACGC554050.032.1378254
TATCAAC572000.031.1564792
CAACGCA609450.029.2389565
AACGCAG638100.027.9899066
ACGCAGA674000.025.9009867
GTGGTAT98950.025.7639641
CGCAGAG672050.025.7401988
GTACATG604100.024.9210191
TACATGG599350.024.3788242
TGGTATC103750.023.4356352
GCAGAGT719750.023.4023539
ACATGGG619150.022.746273
CATGGGA286100.020.756224
CAGAGTA702350.019.51539410-11
AGAGTAC672650.019.26774810-11
GAGTACT371150.019.19745412-13
CATGGGG279700.019.05114
Produced by FastQC (version 0.11.5)