FastQCFastQC Report
Thu 2 Jun 2016
DRR015308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12821890
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246340.19212456197955216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214480.16727643116576418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243850.054.881671
GTATCAA459500.040.6836741
ATCAACG573050.032.1667753
TCAACGC583850.031.6496734
TATCAAC595550.031.1752152
CAACGCA618000.029.8639435
AACGCAG649200.028.4637456
GTGGTAT98650.026.2732331
ACGCAGA714300.025.4429057
CGCAGAG708900.025.3607778
GTACATG627800.024.4660911
TACATGG623150.023.9684182
TGGTATC103650.023.1753182
GCAGAGT762550.022.997919
ACATGGG638950.022.6051543
CATGGGG301800.020.0174834
CAGAGTA752250.019.2739910-11
AGAGTAC720650.019.22271210-11
CATGGGA280800.019.1816184
GAGTACT408400.018.97944612-13
Produced by FastQC (version 0.11.5)