FastQCFastQC Report
Thu 2 Jun 2016
DRR015307_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015307_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12289921
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243680.19827629485982862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222750.1812460796127168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207700.052.9245221
GTATCAA393200.041.2975231
ATCAACG482700.033.072153
TCAACGC488350.032.718144
TATCAAC511250.031.463262
CAACGCA511450.031.285535
AACGCAG541800.029.6919986
GTGGTAT89100.029.2330761
TGGTATC91200.026.5664272
ACGCAGA600900.026.4768127
CGCAGAG601000.026.336798
GCAGAGT638350.024.304739
GTACATG389900.023.3812121
TACATGG384850.022.6144262
ACATGGG395200.020.9572643
GAGTACT413200.020.2549512-13
AGAGTAC603300.020.0907510-11
CAGAGTA633350.020.02471510-11
AGTACTT422250.019.06118412-13
GTACTTT440700.018.7172514-15
Produced by FastQC (version 0.11.5)