FastQCFastQC Report
Thu 2 Jun 2016
DRR015307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12289921
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278220.22638062522940547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257460.20948873471196439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140950.11468747439466861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232300.054.4490431
GTATCAA424950.040.0649871
ATCAACG528450.031.7040733
TCAACGC533650.031.429244
TATCAAC551950.030.5211772
CAACGCA551700.030.4669325
GTGGTAT92050.029.292841
AACGCAG581100.029.0272626
TGGTATC97150.026.4109252
ACGCAGA651300.025.6191967
CGCAGAG648000.025.4900268
GCAGAGT691550.023.4112829
GTACATG398650.023.205011
TACATGG391450.022.4764392
ACATGGG411700.020.5959723
GAGTACT467300.020.37478312-13
CAGAGTA683100.020.22540310-11
AGAGTAC655600.019.67557310-11
GTACTTT485700.019.2108214-15
AGTACTT476150.018.6200112-13
Produced by FastQC (version 0.11.5)