FastQCFastQC Report
Thu 2 Jun 2016
DRR015306_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015306_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16349664
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217890.13326879378071624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192850.11795349433480712No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA236900.048.6638571
GTATCAA413700.036.7264181
ATCAACG497250.030.1427383
TCAACGC501050.029.9419274
TATCAAC528550.028.5694262
CAACGCA545000.027.5443825
AACGCAG572750.026.3127216
GTACATG504050.025.4009271
TACATGG511900.024.001032
ACGCAGA625750.023.6078727
CGCAGAG625150.023.3399268
ACATGGG529400.022.6050643
GTGGTAT117500.022.3199121
GCAGAGT682250.020.8199359
TGGTATC125950.019.4155252
GAGTACA376100.018.849211
CATGGGG361350.018.838464
CAGAGTA650300.018.40060210-11
AGAGTAC606550.018.31387310-11
GAGTACT393450.018.28814712-13
Produced by FastQC (version 0.11.5)