FastQCFastQC Report
Thu 2 Jun 2016
DRR015306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16349664
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252190.15424781818146233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229120.14013743646352608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA258250.050.0962371
GTATCAA456900.038.2658121
ATCAACG562100.030.633083
TCAACGC569900.030.2138164
TATCAAC592250.029.2603022
CAACGCA595750.028.8875475
AACGCAG628900.027.3937846
GTACATG525650.025.4353981
ACGCAGA703350.023.9831687
TACATGG532250.023.959642
CGCAGAG701800.023.718518
GTGGTAT129300.022.4775641
ACATGGG561000.022.0325683
GCAGAGT751850.021.431729
TGGTATC134800.020.4866832
AGAGTAC674950.019.12238310-11
CATGGGG382200.018.8777754
CAGAGTA719750.018.8644510-11
GAGTACA391600.018.8102461
GAGTACT448100.018.79052212-13
Produced by FastQC (version 0.11.5)