FastQCFastQC Report
Thu 2 Jun 2016
DRR015305_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015305_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12482981
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260310.20853192038023607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230500.184651406583091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134270.1075624484247793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200800.054.667351
GTATCAA383700.040.9968721
ATCAACG473250.032.9416733
TCAACGC479700.032.4228554
TATCAAC494600.031.5163672
CAACGCA502400.030.985935
AACGCAG532050.029.3613136
GTGGTAT90950.028.3340231
TGGTATC89600.027.0387742
ACGCAGA585900.026.2155027
CGCAGAG581100.026.1820328
GTACATG395500.025.5219421
TACATGG391800.024.6989142
GCAGAGT621900.023.8796529
ACATGGG404800.022.8980983
CAGAGTA610850.020.2001810-11
AGAGTAC585650.020.012810-11
GAGTACT415250.019.83171812-13
CATGGGG211000.018.5681924
GTACTTT444050.018.39160714-15
Produced by FastQC (version 0.11.5)