FastQCFastQC Report
Thu 2 Jun 2016
DRR015305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12482981
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299540.23995870858090707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278630.22320790202276206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159090.12744551962387832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223950.057.4825631
GTATCAA420500.040.3421171
ATCAACG525850.031.8619043
TCAACGC530650.031.5051144
TATCAAC546800.030.7603362
CAACGCA548950.030.4631355
AACGCAG579150.028.968886
TGGTATC88450.028.1872622
GTGGTAT93000.028.06641
ACGCAGA644800.025.610237
CGCAGAG639950.025.5982768
GTACATG406250.025.2068141
TACATGG403150.024.4434192
GCAGAGT685550.023.3250899
ACATGGG412600.022.9994243
GAGTACT468900.020.37811712-13
CAGAGTA674150.020.3293410-11
AGAGTAC647100.020.02615510-11
GTACTTT497050.019.0783914-15
AGTACTT484200.018.85555512-13
Produced by FastQC (version 0.11.5)