FastQCFastQC Report
Thu 2 Jun 2016
DRR015304_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015304_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9499254
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232070.2443033947718421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213320.2245650026833686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120710.12707313647998042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167700.055.5458561
GTATCAA312250.039.6447681
ATCAACG381900.032.158733
TCAACGC386000.031.829274
TATCAAC399200.030.786652
CAACGCA403550.030.433935
AACGCAG421750.029.1848746
GTGGTAT69450.029.1720431
TGGTATC67450.028.2866922
ACGCAGA466250.026.199937
CGCAGAG467650.025.9178778
GTACATG293500.024.1988981
GCAGAGT499800.023.805149
TACATGG289750.023.2902872
ACATGGG296250.021.7342053
CAGAGTA502850.020.38534410-11
AGAGTAC481600.020.0188810-11
GAGTACT362650.019.9984312-13
GTACTTT382200.018.77301414-15
AGTACTT371950.018.72164712-13
Produced by FastQC (version 0.11.5)