FastQCFastQC Report
Thu 2 Jun 2016
DRR015304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9499254
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261990.2758006049738222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249650.26281011119399483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144850.1524856583474871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183500.058.609621
GTATCAA347400.039.900761
TGGTATC66600.032.4475332
ATCAACG432650.031.7231083
TCAACGC437250.031.45184
CAACGCA449000.030.6287295
TATCAAC451100.030.5181642
GTGGTAT74800.030.266611
AACGCAG465500.029.5919326
ACGCAGA522150.026.093887
CGCAGAG524300.025.8740798
GCAGAGT559000.023.9181299
GTACATG295950.023.872751
TACATGG296050.022.712852
ACATGGG304350.021.2850073
CAGAGTA555700.020.83522610-11
AGAGTAC536900.020.32767510-11
GAGTACT414450.020.32673312-13
GTACTTT435150.019.20426614-15
AGTACTT425100.019.00402512-13
Produced by FastQC (version 0.11.5)