FastQCFastQC Report
Thu 2 Jun 2016
DRR015303_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015303_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7064655
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322230.456115691424422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271540.3843641338465926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160980.2278667535782002No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.10109481637815293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188050.059.3485451
GTATCAA352050.043.489211
GTGGTAT63250.036.2983861
TGGTATC63200.035.976672
ATCAACG464450.032.630163
TCAACGC468750.032.3310664
TATCAAC473750.032.0474322
CAACGCA493200.030.709175
AACGCAG512700.029.6024746
ACGCAGA562950.026.613037
CGCAGAG570100.026.160488
GCAGAGT587600.024.8405889
GTACATG388550.024.151141
TACATGG370050.023.8017522
ACATGGG396800.021.6887153
CAGAGTA568450.021.53125610-11
GAGTACT359900.021.47271212-13
GTACTTT371250.020.63225714-15
AGAGTAC556400.020.61555710-11
AGTACTT358100.020.50701112-13
Produced by FastQC (version 0.11.5)