FastQCFastQC Report
Thu 2 Jun 2016
DRR015303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7064655
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364480.5159204518833546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314820.4456268565131631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182410.258200860480802No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT86070.1218318516615461No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83330.11795338908977154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205600.059.8001941
GTATCAA388450.043.2961581
GTGGTAT68650.037.2922481
TGGTATC71000.035.178222
ATCAACG511500.032.638343
TCAACGC516750.032.3155484
TATCAAC521750.032.062032
CAACGCA535350.031.1333085
AACGCAG553050.030.2109346
ACGCAGA617700.026.8280537
CGCAGAG625300.026.286168
GCAGAGT650750.024.8879749
GTACATG396150.023.147651
TACATGG378200.022.5788942
GAGTACT402250.022.43160412-13
CAGAGTA630100.021.7808710-11
GTACTTT417950.021.31793214-15
AGTACTT402900.020.98370412-13
AGAGTAC614150.020.7389810-11
ACATGGG403350.020.6045973
Produced by FastQC (version 0.11.5)