FastQCFastQC Report
Thu 2 Jun 2016
DRR015302_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015302_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9478123
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327560.345595852680958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277750.2930432533952134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151810.16016884355689412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221900.058.377611
GTATCAA424750.043.6343271
ATCAACG555600.033.0991943
TCAACGC560000.032.8721544
TATCAAC566150.032.4948432
GTGGTAT79350.031.2266921
CAACGCA600050.030.6026025
AACGCAG621350.029.6118226
TGGTATC83900.028.4543672
ACGCAGA672000.027.0619687
CGCAGAG681500.026.4919558
GCAGAGT709750.024.8809599
GTACATG481850.022.8475061
TACATGG467900.022.3854352
GAGTACT402850.021.66365812-13
CAGAGTA689800.020.99314710-11
AGAGTAC662200.020.75152610-11
ACATGGG495700.020.4544123
AGTACTT406550.020.36407512-13
GTACTTT425450.020.15443814-15
Produced by FastQC (version 0.11.5)