FastQCFastQC Report
Thu 2 Jun 2016
DRR015302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9478123
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314840.3321754739836147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270300.2851830473185461No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT173710.18327468423864093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145880.15391233053211065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228950.058.147291
GTATCAA430050.042.8855971
GTGGTAT83300.032.980881
ATCAACG557000.032.871863
TCAACGC563350.032.5094074
TATCAAC570650.032.1605262
CAACGCA586600.031.2053815
AACGCAG608500.030.1570536
TGGTATC89200.029.5304992
ACGCAGA680200.026.7310287
CGCAGAG687900.026.2957158
GCAGAGT714250.024.7969429
GTACATG486800.023.1172561
TACATGG474850.022.4956592
GAGTACT406450.021.9197112-13
CAGAGTA695100.021.1710410-11
GTACTTT426450.020.51470814-15
ACATGGG504400.020.4992473
AGAGTAC670750.020.47108510-11
AGTACTT414800.020.28277812-13
Produced by FastQC (version 0.11.5)