FastQCFastQC Report
Thu 2 Jun 2016
DRR015301_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015301_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6263130
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT584980.9340058405302141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478900.7646336576120886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288430.4605205384528183No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA128100.2045303226980759No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA110250.17603019576473744No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103640.16547636724768605No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA103580.16538056850169164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82550.1318031080306492No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT80410.12838628609018174No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC80340.1282745208865216No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA79820.12744426508790332No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC72790.11621984534889104No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG72080.1150862268546238No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA67120.10716686385241884No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA65110.10395760586160595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62790.10025338768315524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA288050.057.6681671
GTATCAA552050.043.5801051
ATCAACG740700.032.366313
TATCAAC743200.032.236592
TCAACGC753500.031.822794
GTGGTAT101300.029.765751
TGGTATC103100.028.89822
CAACGCA830700.028.826345
AACGCAG850750.028.275136
ACGCAGA882300.027.142987
CGCAGAG881850.027.0555958
GCAGAGT909600.025.7670679
TATAACG7100.025.665712
AATGCGG24050.024.9698987
CATGGGT101300.023.8998664
TACATGG595650.023.66362
GTACATG615400.023.5806921
GAGTACT515500.023.40363712-13
AGTACTT517600.022.81640212-13
GCGTATC1802.9293187E-722.7739471
Produced by FastQC (version 0.11.5)