FastQCFastQC Report
Thu 2 Jun 2016
DRR015301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6263130
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640231.0222205191334046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542440.8660845296201739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322260.5145350647360025No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT273220.4362355563432342No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT148990.237884252761798RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG120480.1923638819567852RNA PCR Primer, Index 39 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117000.18680755468910912No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT87890.14032919642415215No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG86270.13774263028230294No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG85120.1359064876507433No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT79720.12728460051124596RNA PCR Primer, Index 39 (96% over 25bp)
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA79070.12624678076297316No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG76430.1220316359392189No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA75150.1199879293580047No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT73980.11811985381111362No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT73320.11706606760517504No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT72940.11645934221387708No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70470.11251562717044034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69020.11020049080890865No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG65290.10424500209958917No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA64580.10311138360532195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA313200.059.785481
GTATCAA603550.043.4191471
GTGGTAT103350.032.7091371
TATCAAC804850.032.5362362
ATCAACG805200.032.507683
TGGTATC103950.032.2993162
TCAACGC820300.031.8815564
CAACGCA864700.030.2129555
AACGCAG884800.029.578026
ACGCAGA960700.027.0470737
CGCAGAG958550.026.9918988
GCAGAGT992750.025.7687389
TCTGTCG12400.023.8482578
GAGTACT593200.023.6172312-13
GTACATG620850.023.5335751
TACATGG603250.023.5072022
CATGGGT100950.023.3873424
AGTACTT596850.022.46268512-13
ACATGGG623900.022.2457523
GTACTTT633950.021.99698814-15
Produced by FastQC (version 0.11.5)