FastQCFastQC Report
Thu 2 Jun 2016
DRR015300_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015300_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8321551
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335530.40320608501948735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275060.3305393429662331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162700.19551643677963398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231250.050.755241
GTATCAA415950.041.1822851
ATCAACG536900.031.7060893
TCAACGC538250.031.6363544
TATCAAC550150.030.9556392
CAACGCA571350.029.8832115
AACGCAG588550.029.2179496
ACGCAGA643300.026.4930577
CGCAGAG654650.025.9590028
GCAGAGT678300.024.4911779
GTGGTAT101750.024.1269571
GTACATG440650.023.555851
TACATGG424850.023.1525382
TGGTATC105400.022.261182
GAGTACT375300.022.21671312-13
ACATGGG450350.021.228743
AGAGTAC611200.020.97485210-11
AGTACTT377250.020.95004512-13
GTACTTT395200.020.89045714-15
CAGAGTA660850.020.66931710-11
Produced by FastQC (version 0.11.5)