FastQCFastQC Report
Thu 2 Jun 2016
DRR015300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8321551
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370100.4447488214636911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313520.37675668874708573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180640.21707491788489913No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT131590.1581315790770254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA247400.051.2126161
GTATCAA447100.040.566091
ATCAACG571200.031.4739723
TCAACGC576200.031.177174
TATCAAC587300.030.6843722
CAACGCA594950.030.164035
AACGCAG613750.029.306776
ACGCAGA687750.025.922257
CGCAGAG695400.025.4756838
GTGGTAT110300.024.3285061
GCAGAGT723000.024.1130289
TGGTATC111350.023.4527652
GTACATG443050.023.4454081
TACATGG426950.023.0383342
GAGTACT414400.022.05312512-13
TATAACG7950.021.7464732
ACATGGG454050.021.0596853
GTACTTT438450.020.65327514-15
AGAGTAC650600.020.61886210-11
AGTACTT418900.020.54537412-13
Produced by FastQC (version 0.11.5)