FastQCFastQC Report
Thu 2 Jun 2016
DRR015299_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015299_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7623368
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330590.4336534717988165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265390.34812696960188727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161330.21162562269065327No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180100.058.259561
GTATCAA354750.044.391621
ATCAACG455550.034.2823143
TCAACGC461850.033.82524
GTGGTAT64800.033.312081
TATCAAC471950.033.1933942
CAACGCA488850.031.9760115
AACGCAG503750.031.16476
TGGTATC69950.029.5002632
ACGCAGA554350.028.1499587
CGCAGAG563000.027.5501128
GCAGAGT586350.026.1901959
GTACATG381550.025.554281
TACATGG372050.025.0751822
TATAACG6350.024.3904932
ACATGGG394450.023.014943
GAGTACT365100.021.93395612-13
CAGAGTA574200.021.66888610-11
CATGGGT80450.021.6640784
AGAGTAC556300.021.24147810-11
Produced by FastQC (version 0.11.5)