FastQCFastQC Report
Thu 2 Jun 2016
DRR015299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7623368
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379870.498296815790606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314860.413019547265723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189720.24886638031904007No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT96050.12599418000023085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79560.1043633207789523No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204300.058.3119621
GTATCAA397150.043.8764231
ATCAACG508050.034.000193
TCAACGC515650.033.4814264
GTGGTAT73500.033.408411
TATCAAC521050.033.233532
CAACGCA534450.032.2525985
AACGCAG552850.031.2285396
TGGTATC75550.031.134592
ACGCAGA615950.027.8595057
CGCAGAG620200.027.4721668
GCAGAGT650300.025.9487179
GTACATG393350.023.3836081
TACATGG383100.022.8734262
GAGTACT415850.022.59393112-13
CAGAGTA635700.021.81823710-11
ACATGGG398750.021.3461883
GTACTTT436650.021.27932414-15
TATAACG7500.021.2321432
AGAGTAC617950.021.02395610-11
Produced by FastQC (version 0.11.5)