FastQCFastQC Report
Thu 2 Jun 2016
DRR015298_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015298_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9232265
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293750.31817760863666716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245330.26573110715517806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135530.14680037888860425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199250.056.530061
GTATCAA381850.043.715441
ATCAACG488550.034.0162773
TCAACGC494150.033.621944
GTGGTAT73600.032.7983321
TATCAAC509900.032.6103742
CAACGCA532050.031.2178555
AACGCAG548250.030.377596
TGGTATC78850.029.0612472
ACGCAGA599050.027.6067057
CGCAGAG609600.027.0038098
GCAGAGT632500.025.5159389
GTACATG482050.023.9518781
TACATGG460200.023.322582
ACATGGG492100.021.3611013
CAGAGTA623200.021.15104910-11
GAGTACT358650.021.05270412-13
GTACTTT362350.020.55481714-15
AGAGTAC604400.020.38990810-11
AGTACTT352150.020.23324412-13
Produced by FastQC (version 0.11.5)