FastQCFastQC Report
Thu 2 Jun 2016
DRR015298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9232265
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323030.3498924695077535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283030.306566156842335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153890.16668715640203136No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT151340.16392510396961094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214600.057.7977681
GTATCAA405750.043.1695481
GTGGTAT78600.033.56671
ATCAACG529900.032.8541153
TCAACGC534650.032.570744
TATCAAC550600.031.7461512
TGGTATC80150.031.5603052
CAACGCA560450.031.0713625
AACGCAG577200.030.1854526
ACGCAGA642500.026.9122477
CGCAGAG652800.026.3017488
GCAGAGT682500.024.7508359
GTACATG484050.023.233121
TACATGG460350.022.6613542
GAGTACT399650.021.47870612-13
CAGAGTA668500.021.13580910-11
GTACTTT404450.020.9553214-15
ACATGGG491800.020.6087633
AGAGTAC652250.020.38237810-11
AGTACTT395500.020.18542912-13
Produced by FastQC (version 0.11.5)