FastQCFastQC Report
Thu 2 Jun 2016
DRR015297_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015297_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9140595
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341600.3737174658761273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288790.31594223351980916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166900.18259205226793224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204150.057.7805561
GTATCAA384450.043.453111
ATCAACG500800.032.9946483
TCAACGC501750.032.914224
TATCAAC514600.032.295192
CAACGCA533000.030.9846155
AACGCAG551450.030.1451856
GTGGTAT77150.027.2732831
ACGCAGA611300.026.9718487
CGCAGAG621200.026.4412028
TGGTATC78600.025.6184332
GCAGAGT645150.025.0515389
GTACATG434050.024.248871
TACATGG425350.023.4986522
GAGTACT390900.021.67355712-13
CAGAGTA623800.021.41578510-11
ACATGGG453800.021.2996083
AGAGTAC609200.020.73025710-11
AGTACTT394850.020.55790512-13
GTACTTT409100.020.46437614-15
Produced by FastQC (version 0.11.5)