FastQCFastQC Report
Thu 2 Jun 2016
DRR015297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9140595
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372890.40794937309879714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320960.3511368789449702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184510.20185775652460258No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT122940.13449890297075848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226900.058.6271551
GTATCAA416950.042.39341
GTGGTAT79050.034.693261
ATCAACG546600.032.174743
TCAACGC549100.032.019974
TGGTATC84000.031.6846792
TATCAAC562600.031.3118232
CAACGCA570700.030.8558945
AACGCAG591850.029.8301076
ACGCAGA659700.026.6036327
CGCAGAG669400.026.1109688
GCAGAGT701800.024.5038629
GTACATG448900.023.5038721
TACATGG436250.022.787082
GAGTACT428700.022.03934712-13
CAGAGTA681050.021.37386110-11
GTACTTT446500.020.88741714-15
ACATGGG465350.020.831793
AGAGTAC660650.020.43959610-11
AGTACTT434550.020.28196712-13
Produced by FastQC (version 0.11.5)