FastQCFastQC Report
Thu 2 Jun 2016
DRR015296_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015296_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8975935
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475840.5301286161274563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373290.41587868004837375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226630.2524862312394196No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA103730.11556456235478532No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA99610.11097451129046723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA269150.058.1108361
GTATCAA514750.043.1792071
ATCAACG671500.032.8425753
TATCAAC675450.032.6880722
TCAACGC677250.032.5642784
CAACGCA742250.029.6754555
AACGCAG760200.029.093676
ACGCAGA800450.027.389987
GTGGTAT97100.027.252611
CGCAGAG806200.027.0501698
TGGTATC101600.025.561142
GCAGAGT842700.025.4579689
GTACATG600250.024.3190751
TACATGG584400.024.082792
GAGTACT468100.022.74507512-13
ACATGGG609500.022.2730333
AGTACTT467050.021.6515312-13
GTACTTT486200.021.57523514-15
CAGAGTA821200.021.54496410-11
AGAGTAC792700.020.7210110-11
Produced by FastQC (version 0.11.5)