FastQCFastQC Report
Thu 2 Jun 2016
DRR015296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8975935
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524410.5842399705434588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439160.48926379257425545No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT251730.28044989184970703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250980.2796143243015909No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT139070.15493650522201866TruSeq Adapter, Index 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102670.11438362688678115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA294450.057.818391
GTATCAA555750.042.7329331
ATCAACG717500.032.981923
TATCAAC725950.032.6961942
TCAACGC726050.032.5747224
CAACGCA766450.030.8339525
AACGCAG785100.030.1246686
GTGGTAT103100.029.9588761
TGGTATC109450.027.6016712
ACGCAGA865400.027.0560687
CGCAGAG871650.026.7499358
GCAGAGT913250.025.1778689
GTACATG626900.023.2689881
TACATGG610750.022.981312
GAGTACT517400.022.79081712-13
GTACTTT535150.021.79358914-15
CAGAGTA891550.021.50546510-11
ACATGGG638400.021.3916973
GCGTAAT1956.318933E-721.0262761
AGTACTT515100.020.71065312-13
Produced by FastQC (version 0.11.5)