FastQCFastQC Report
Thu 2 Jun 2016
DRR015295_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015295_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8179327
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363550.4444742214121039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281770.34449044524078815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179460.21940680449626235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199100.055.969551
GTATCAA384800.043.101661
TATCAAC499550.033.046752
ATCAACG498650.032.9865843
TCAACGC503350.032.6799744
CAACGCA538050.030.5725615
AACGCAG556800.029.6981376
GTGGTAT79050.027.8825571
ACGCAGA612650.026.836857
CGCAGAG616750.026.4912178
GTACATG455350.025.282551
TGGTATC83900.024.9755652
GCAGAGT649250.024.8229589
TACATGG448650.024.7032382
ACATGGG475600.022.7985443
GAGTACT379700.021.3061712-13
CAGAGTA638400.020.54439510-11
AGAGTAC616450.020.47138410-11
CATGGGG277750.020.3802644
AGTACTT387200.020.3294212-13
Produced by FastQC (version 0.11.5)