FastQCFastQC Report
Thu 2 Jun 2016
DRR015295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8179327
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398420.48710609075783373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324790.3970864595583475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201930.2468785023511103No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT142310.1739874197473704No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT94920.11604866757375026RNA PCR Primer, Index 26 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222400.057.6925961
GTATCAA423500.042.4977461
ATCAACG552050.032.3609963
TATCAAC553500.032.345932
TCAACGC558050.032.0293664
CAACGCA579550.030.8489975
AACGCAG596150.030.020526
GTGGTAT82050.028.8211291
ACGCAGA671450.026.3830077
TGGTATC88900.025.9988672
CGCAGAG677550.025.9921678
GTACATG465850.024.5499081
GCAGAGT705650.024.3962149
TACATGG459700.024.0406112
ACATGGG485300.022.0759813
TACCTGG63350.021.9051232
GAGTACT427750.021.89070712-13
CAGAGTA693150.020.82677710-11
AGTACTT434050.020.5823812-13
GTACTTT453600.020.44877814-15
Produced by FastQC (version 0.11.5)