FastQCFastQC Report
Thu 2 Jun 2016
DRR015294_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015294_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9750728
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342910.3516763056050789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268870.2757435137150785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159860.16394673300291013No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT145820.14954780812263452Illumina RNA PCR Primer (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204950.057.4246181
GTATCAA409900.045.0685461
ATCAACG534050.034.3039323
TCAACGC538700.034.0505874
TATCAAC549250.033.451922
CAACGCA580050.031.5835115
AACGCAG600050.030.5832716
GTGGTAT76750.028.9004171
ACGCAGA648200.028.0336577
CGCAGAG654750.027.5529738
TGGTATC79700.026.9231262
GCAGAGT688000.025.6007889
GTACATG472850.023.70511
TACATGG457050.022.985742
GAGTACT405250.021.46726812-13
ACATGGG483250.021.2996673
CAGAGTA668150.020.99170710-11
AGAGTAC647750.020.97846410-11
AGTACTT407850.020.76145712-13
GTACTTT419200.020.49766214-15
Produced by FastQC (version 0.11.5)