FastQCFastQC Report
Thu 2 Jun 2016
DRR015294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9750728
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377960.3876223395832598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312340.3203248003636241No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT192520.19744166794520368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178730.1832991341774686No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT101110.10369482155588794RNA PCR Primer, Index 17 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217550.057.2716871
GTATCAA424900.044.579941
ATCAACG556150.033.774993
TCAACGC561300.033.562364
TATCAAC575550.032.7019542
AATCGTA8550.032.4591528
CAACGCA588650.031.9720655
AACGCAG606850.031.058176
GTGGTAT80100.029.8585381
TGGTATC82300.028.0824582
ACGCAGA677500.027.5776777
CGCAGAG683900.027.115348
ATCGTAT13100.027.0892379
AAACTCG18200.026.7457436
GCAGAGT717900.025.3368329
ACTCGTA18150.024.314688
GTACATG473000.023.7698141
TACATGG455300.023.1525922
ACATGGG477950.021.330533
GAGTACT435250.021.29436512-13
Produced by FastQC (version 0.11.5)