FastQCFastQC Report
Thu 2 Jun 2016
DRR015293_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015293_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9420734
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301760.32031474405285193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235200.24966207516314548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136350.14473394535924694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203550.054.5967941
GTATCAA380850.042.7055471
ATCAACG491950.032.831233
TCAACGC494350.032.6449134
TATCAAC505600.032.123192
CAACGCA528000.030.5987365
AACGCAG547450.029.6769286
ACGCAGA597650.026.9635357
CGCAGAG608700.026.318498
GTGGTAT85000.025.6664031
GCAGAGT634500.024.6472939
TGGTATC87050.023.8650462
GTACATG461600.023.4339891
TACATGG447400.023.0438352
ACATGGG470500.021.2603383
GAGTACT348250.020.94419712-13
CAGAGTA616750.020.63500810-11
AGAGTAC593700.020.1869210-11
GTACTTT361500.019.93701414-15
AGTACTT350950.019.79771812-13
Produced by FastQC (version 0.11.5)