FastQCFastQC Report
Thu 2 Jun 2016
DRR015293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9420734
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336020.3566813371442183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278530.29565636817683205No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT191300.20306273375301753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155680.16525251641751057No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT98780.10485382561486185TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221050.056.401971
GTATCAA416500.042.7086681
ATCAACG532350.033.2573933
TCAACGC536800.032.9986424
TATCAAC546450.032.5348662
CAACGCA561250.031.5205785
AACGCAG577400.030.6862136
ACGCAGA651050.026.9493267
CGCAGAG659900.026.3564648
TGGTATC86850.025.6667652
GTGGTAT89700.025.645371
GCAGAGT694400.024.6474729
GTACATG472300.022.8870491
TACATGG459150.022.4616572
GAGTACT393850.021.86936212-13
CAGAGTA672650.021.03212710-11
GTACTTT407750.020.9581314-15
ACATGGG486600.020.7722993
AGTACTT396250.020.28433212-13
AGAGTAC647650.020.15800910-11
Produced by FastQC (version 0.11.5)