FastQCFastQC Report
Thu 2 Jun 2016
DRR015292_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015292_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8428660
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310430.3683029093592576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246870.2928935323052538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146820.17419138985319138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185800.055.574141
GTATCAA360700.043.4515721
ATCAACG461550.033.59283
TCAACGC464350.033.4004334
TATCAAC482450.032.2114262
CAACGCA492350.031.5198085
AACGCAG509700.030.4909866
ACGCAGA557900.027.621257
CGCAGAG568350.026.9382368
GTGGTAT76050.025.3941421
GCAGAGT594200.025.1780629
TGGTATC77300.024.2238352
GTACATG413850.024.190881
TACATGG401050.023.4472392
TATAACG8000.022.7798332
TACCTGG65850.021.7939192
ACATGGG426150.021.304593
GAGTACT353900.021.18822912-13
AGTACTT356300.021.04550612-13
AGAGTAC561100.021.02321210-11
Produced by FastQC (version 0.11.5)