FastQCFastQC Report
Thu 2 Jun 2016
DRR015292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8428660
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340690.4042042270064281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286200.33955575382089204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167780.1990589251434985No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT119320.14156461406676743No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210000.056.6425131
GTATCAA395850.042.6165241
ATCAACG509350.033.019223
TCAACGC512800.032.805944
TATCAAC522350.032.1904562
CAACGCA535300.031.4100285
AACGCAG551850.030.5339896
GTGGTAT75650.030.1103651
TGGTATC81500.027.4640332
ACGCAGA615550.027.226387
CGCAGAG626050.026.6411618
GCAGAGT653550.024.9913949
GTACATG423800.023.735211
TACATGG405250.023.3730032
GAGTACT408200.021.61322612-13
ACATGGG433500.021.376453
CAGAGTA642200.021.16515510-11
TACCTGG64450.020.611842
GTACTTT424350.020.5466714-15
AGAGTAC624050.020.50851610-11
Produced by FastQC (version 0.11.5)